Remember that 4 transfers is based on 1 transfer every 2 days. So if you do a transfer every day, you need 8 transfers, not 4. If you have several new fish, ammonia may build up fast, so transfer every 2 days may be too long. The maximum time between transfers is based on the minimum life cycle. every 48 hours < minimum 3 day life cycle. Going to every 3 days is high risk for both ich and ammoinia. The total number of transfers is based on the maximum life cycle. every 2 days * 4 transfers = 8 days > maximum 7 life cycle. every 1 days * 8 transfers = 8 days > maximum 7 life cycle. Side note: Quite a few people use Transfer #2 to treat with PraziPro. Not for ich of course, but for fluke and other parasites. Some also do dips/baths when you are moving the fish between buckets. Particularly for clownfish and brook. But you have to be careful not to over-stress the fish.