reefermadness
Guest
well, rinsing in tap water will kill 100% of the bacteria due to chlorine and chloramines. i know the same bacteria is found in fresh and saltwater. its all the same bacteria as far as the type (nitrosomonas and Nitrobacter). so not sure how much will be killed in ro/di. if the sand is still damp, and fresh from another good running tank, why clean it? maybe stir it up in a bucket of salt water to remove any excess detritus. but, on the same note, if you are going to add l/r then l/s isnt necessary as it will re-grow the bacteria. unless you are trying to jump start a system with alot of bacteria, then there is no reason to worry about the loss of bacteria in ur sand from washing it. but i wouldnt rinse it in anything but ro/di, altho saltwater would be the best solution regardless.