Kessil

Microscope images of reef organisms

tribbitt

Supporting Member
My wonderful AP Biology teacher gave me a microscope. Of course, some of the first things I took a look at are reef organisms. I am open to suggestions of other things to look at!

Because there's so many endosymbiotic photosynthesizers in the marine environment, I picked a red photosynthetic flatworm, a tentacle of a F. paraancora, and a photosynthetic sponge. Also, a root from a Salvinia minima floating plant in my freshwater tank.

Flatworms not under microscope:

IMG_4074.jpeg


Flatworms under microscope at 400x, 100x, 40x. The green circles are its symbiotic alga, which was really cool to see. I didn't expect its tissue to be so vacuolated or to have the texture and organization it does.
AcoelFlatworm.png



This is a side-on view of F. paraancora "bicolor hammer" tentacle at 400x. The golden-brown circles are the zooxanthellae: dinoflagellates in the genera Symbiodinium, Cladocopium, prob others as well
IMG_4079.jpeg



This is a piece of photosynthetic purple sponge from @psidriven at 400x. Not the best slide but it was tough to get a good thin sample.

I know the compression massacred it, but the pink noise is the symbiotic cyanobacteria which perform photosynthesis for the sponge. Despite being "cyan"obacteria, these ones produce red phycoerythrins. Under sunlight it looks blue-violet and not red, so I'm guessing the sponge produces iridophores to protect itself from harsh wavelengths?

Also there's some large cells or bundles of cells scattered throughout. I think that might be the sponge itself?

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In comparison, plant cells (especially root cells) are massive! These are Salvinia minima roots at 400x.

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Again if you have some other cool organisms in mind, I'm open to suggestions!
 
Diatoms, diatoms, diatoms!
Under low magnification: fish scale (from market seafood). So many algae - so different. Sloughed off coral tissue. Shine actinic light on live coral tissue/zooxanthellae while viewing.
While looking at algae, change salinity drastically.
If you have access to low magnification stereo/dissecting microscope: urchin spine vs. shrimp antenna, coral skeleton, asterina tube feet, tiniest shells you can find. Nature's patterns, not even meant for our eyes.
Any type of plankton - zooplankton moving, larvae. Living rotifers - my favorite! Micro worms.
Foraminiferans. I don't have any now, but bioluminescent dinoflagellates.
Swish a soft coral, bit of live rock, or a pinch of sand in a bit of SW and be surprised at what you observe.
 
Do you have access to live brine shrimp? Tiny single LED blacklight? I might be able to grab some fluorescent microplastic from my school. A fascinating (but also depressing) observation of bioaccumulation of microplastics in the ocean. :(
Oh yah - if your school has any oleophilic bacteria - oil-eating microbes - that could be an interesting view
 
Diatoms, diatoms, diatoms!
Under low magnification: fish scale (from market seafood). So many algae - so different. Sloughed off coral tissue. Shine actinic light on live coral tissue/zooxanthellae while viewing.
While looking at algae, change salinity drastically.
If you have access to low magnification stereo/dissecting microscope: urchin spine vs. shrimp antenna, coral skeleton, asterina tube feet, tiniest shells you can find. Nature's patterns, not even meant for our eyes.
Any type of plankton - zooplankton moving, larvae. Living rotifers - my favorite! Micro worms.
Foraminiferans. I don't have any now, but bioluminescent dinoflagellates.
Swish a soft coral, bit of live rock, or a pinch of sand in a bit of SW and be surprised at what you observe.

Definitely wanted to do diatoms but could not find any. Maybe I’ll dose a bunch of silicate to get it to happen.

I did a piece of hammer coral already but I do want to see more.

Thanks for all the suggestions I’ll work through them slowly!
 
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